This textbook for graduate veterinary students introduce the concept, objectives, and definition of One Health and discuss the advancements made and challenges in One Health around endemic and emerging zoonotic diseases. It provides a framework of basic principles of zoonoses, prevention, control, and eradication. It presents diverse group of zoonoses, including viral, bacterial, rickettsia, fungal, and parasitic. It discusses the vital steps on One health in recognizing the critical importance of collaborative, cross-disciplinary approaches for responding to emerging and resurging diseases, and emphasize wildlife health as an essential component of global disease prevention, surveillance, control, and mitigation. Further, the book examines the potential of new emerging zoonotic disease as a tool for bioterrorism. It also explores the applications and advancements of bioinformatics in veterinary research. This textbook is an essential source for students of veterinary sciences, and also for public health professionals, veterinarians, and physicians. This textbook for graduate veterinary students introduce the concept, objectives, and definition of One Health and discuss the advancements made and challenges in One Health around endemic and emerging zoonotic diseases. It provides a framework of basic principles of zoonoses, prevention, control, and eradication. It presents diverse group of zoonoses, including viral, bacterial, rickettsia, fungal, and parasitic. It discusses the vital steps on One health in recognizing the critical importance of collaborative, cross-disciplinary approaches for responding to emerging and resurging diseases, and emphasize wildlife health as an essential component of global disease prevention, surveillance, control, and mitigation. Further, the book examines the potential of new emerging zoonotic disease as a tool for bioterrorism. It also explores the applications and advancements of bioinformatics in veterinary research. This textbook is an essential source for students of veterinary sciences, and also for public health professionals, veterinarians, and physicians Dr. Ripan Biswas is working as Assistant Professor in Veterinary Public Health in West Bengal University of Animal and Fishery Sciences, Kolkata, India. Dr. Biswas is committed with teaching for last three years and having research interest in food borne bacterial infections, zoonotic diseases, wild life, bacterial biofilm and One Health practice. Previously he served as Veterinary Officer under Government of West Bengal (India) for nearly one decade and he worked with domesticated animals, wild animal and birds. Dr. Biswas has published numerous national and international research articles and review articles in the peer-reviewed national and international journals. He has also published two monographs, two manuals and several popular articles as well as scientific articles. He is a life member of various scientific societies and organizations like Indian Association of Veterinary Public Health Specialists and Association of Public Health Veterinarians, India. Dr. Biswas actedas reviewer for reputed national and international journals. Dr. Ajay Pratap Singh has received a B.V.Sc. & A.H. degree in 2007 from Kerala Agricultural University, Masters in Veterinary Science in 2009, and Ph.D. in 2015 both in Veterinary Bacteriology from the Indian Veterinary Research Institute, Bareilly, India. Since 2011, he works in the Department of Veterinary Microbiology, College of Veterinary Sciences and AH, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidhyalay Evam Go-Anusandhan Sansthan, Mathura as Assistant Professor. His current research is focused on the epidemiological investigation of livestock-associated antimicrobial drug resistance, the molecular mechanism of transmission, and its mitigation strategies. He has completed 7 research projects as principal investigator and/or co-investigator. He has (co-)authored more than 40 peer-reviewed publications in peer-reviewed journals and several invited papers in conferences and book chapters. In addition, he is also acting as reviewer in some leading journals like Frontiers in Immunology, Indian Journal of Animal Science and Indian Journal of Animal Research etc. He has delivered a talk and invited seminars at several workshops and national/international conferences. He is a Life member of various organizations such as the Indian Society for Association of Public health Veterinarians (APHV), the Indian Association of Veterinary Microbiologists, Immunologists, and Specialists in Infectious Diseases (IAVMI), Indian Society of Veterinary Immunology and Biotechnology. Dr. Maninder Singh is Associate Professor in Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India. He has earlier served as Assistant Professor in SKUAST-Jammu (2010-2021). His research interest is